Association of mthfr, mtrr, and rad54l gene variations with meningioma and their correlation with the tumor’s histopathological characteristics in turkish cohort

AIM To elucidate the association of MTHFR, MTRR, and RAD54L gene variations with meningioma in Turkish cohort. MATERIAL AND METHODS DNAs were isolated from 87 retrospective samples. The hotspot regions amplified specific primers via polymerase chain reaction (PCR), next-generation sequencing (NGS) was performed. All detected single-nucleotide polymorphisms (SNPs) listed compared healthy control frequencies different genomic databases. histopathological characteristics meningiomas compared. Pearson?s chi-squared test used to detect statistical differences SNPs, correlation analysis conducted. RESULTS rs1801131, rs1801133, rs4846051 on rs1801394 rs1048771 found be significantly altered overall cohort patients meningioma. frequency rs18011031 is 0.09 cohort, which correlated WHO tumor grades (p = 0.038). rs18011033 0.29 0.045). Furthermore, 0.18 0.023) also low Ki67 proliferation index 0.00455). 0.15 associated high 0.0144). non-necrotic form 0.05). CONCLUSION We reported a significant between genetic alterations folate metabolism (MTHFR, MTRR) DNA repair mechanism (RAD54L) genes Five SNPs these four correlations identified. This preliminary promising study conducted establish marker for diagnosis prognosis